Description |
EGFR Mouse Monoclonal Antibody(REK0116) |
Cat. No. |
REK0116 |
Immunogen |
synthetic peptide coupled to KLH. |
Concentration |
1ug/ul |
Specificity |
The EGFR mouse monoclonal antibody can detect endogenous EGFR proteins. |
MW |
N/A |
Substrate/Buffer |
Phosphate buffered saline (PBS) with containing 0.02% sodium azide. pH 7.2. |
Application |
WB 1:5000 (Optimal dilutions should be determined by the end user) |
Notes |
The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascadesβ-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Actin may not be stable in certain cells. For example, expression of β-Actin in adipose tissue is very low and therefore β-Actin should not be used as loading control for these tissues. |
Storage |
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
Purity |
Immunogen affinity purified |
Clonality |
Monoclonal |
Isotype |
IgG1 |
Alternative names |
Epidermal growth factor receptor antibody, erbb 1 antibody, Receptor tyrosine protein kinase ErbB 1 antibody |
IHC-EGFR(REK0116)Immunohistochemical analysis of paraffin-embedded Human Lung caricnoma using EGFR Mouse mAb diluted at 1:500. |
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IF-EGFR(REK0116)IF analysis of Hela with REK0116(Left) and DAPI (Right) diluted at 1:100. |
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Western Blot-EGFR(REK0116)
Western blot analysis of Hela with EGFR mAb diluted at 1:1,000. |