Description |
EGFR Mouse Monoclonal Antibody(REK0115) |
Cat. No. |
REK0115 |
Immunogen |
synthetic peptide coupled to KLH. |
Concentration |
1ug/ul |
Specificity |
The EGFR antibody can recognize endogenous EGFR proteins. |
MW |
N/A |
Substrate/Buffer |
Phosphate buffered saline (PBS) with containing 0.02% sodium azide. pH 7.2. |
Application |
WB 1:5000 (Optimal dilutions should be determined by the end user) |
Notes |
The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. |
Storage |
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
Purity |
Immunogen affinity purified |
Clonality |
Monoclonal |
Isotype |
IgG1 |
Alternative names |
Epidermal growth factor receptor antibody, erbb 1 antibody, Receptor tyrosine protein kinase ErbB 1 antibody |
IF-EGFR(REK0115)IF analysis of Hela with REK0115(Left) and DAPI (Right) diluted at 1:100. |
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IP-EGFR(REK0115)1、Input: Hela Cell Lysate 2、IP product: IP dilute 1:200 Western blot analysis: primary antibody : REK0115 1:1,000 Secondary antibody: Goat anti-Mouse IgG, Heavy Chain Specific(S003), 1:5,000 |
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Western Blot-EGFR(REK0115)Western blot analysis of 1) A431 ,2) Hela, with REK0115 diluted at 1:2,000. |