Description |
β-tubulin Mouse Monoclonal Antibody(REK0010) |
Cat. No. |
REK0010 |
Immunogen |
synthetic peptide coupled to KLH. |
Concentration |
1ug/ul |
Specificity |
The β-tubulin mouse monoclonal antibody can detect endogenous β-tubulin proteins. |
MW |
N/A |
Substrate/Buffer |
Phosphate buffered saline (PBS) with containing 0.02% sodium azide. pH 7.2. |
Application |
WB 1:5000 (Optimal dilutions should be determined by the end user) |
Notes |
Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues. |
Storage |
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
Purity |
Immunogen affinity purified |
Clonality |
Monoclonal |
Isotype |
IgG1 |
Alternative names |
beta tubulin antibody, TUBB antibody |
IF-β-tubulin(REK0010)IF analysis of Hela with TDY043(Left) and DAPI (Right) diluted at 1:100. |
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IHC-β-tubulin(REK0010)IHC Staining of Human colon tissue with β-tubulin mouse mAb(5G3) diluted at 1:200. |
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Western Blot-β-tubulin(REK0010)Western blot analysis of A549 (1) , Rat brain (2) ,Mouse brain (3) ,Chicken lung (4) and Rabbit testis (5),Sheep muscle (6) with β-tubulin mouse mAb(5G3) diluted at 1:5000. |