Description

β-tubulin Mouse Monoclonal Antibody(REK0010)

Cat. No.

REK0010

Immunogen

 synthetic peptide coupled to KLH.

Concentration

1ug/ul

Specificity

The β-tubulin mouse monoclonal antibody can detect endogenous β-tubulin proteins.

MW

N/A

Substrate/Buffer

Phosphate buffered saline (PBS) with containing 0.02% sodium azide. pH 7.2.

Application

WB 1:5000 

 (Optimal dilutions should be determined by the end user)

Notes

Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues. 

Storage

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Purity

Immunogen affinity purified

Clonality

Monoclonal

Isotype

IgG1

Alternative names

beta tubulin antibody, TUBB antibody



 

Porduct images

 


 

IF-β-tubulin(REK0010)

IF analysis of Hela with TDY043(Left) and DAPI (Right) diluted at 1:100.

 

IHC-β-tubulin(REK0010)

IHC Staining of Human colon tissue with β-tubulin mouse mAb(5G3) diluted at 1:200.

Western Blot-β-tubulin(REK0010)

Western blot analysis of A549 (1) , Rat brain (2) ,Mouse brain (3) ,Chicken lung (4) and Rabbit testis (5),Sheep muscle (6) with β-tubulin mouse mAb(5G3) diluted at 1:5000.