Description

GAPDH Mouse Monoclonal Antibody(REK0005)

Cat. No.

REK0005

Immunogen

 synthetic peptide coupled to KLH.

Concentration

1ug/ul

Specificity

The GAPDH mouse monoclonal antibody can detect endogenous GAPDH proteins.

MW

N/A

Substrate/Buffer

Phosphate buffered saline (PBS) with containing 0.02% sodium azide. pH 7.2.

Application

WB 1:5000 

 (Optimal dilutions should be determined by the end user)

Notes

Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types. 

Storage

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Purity

Immunogen affinity purified

Clonality

Monoclonal

Isotype

IgG1

Alternative names

G3PDH antibody, Glyceraldehyde 3 phosphate dehydrogenase antibody, MGC102544 antibody


 

Porduct images

 


 

Western Blot-GAPDH(REK0005)-2

Western blot analysis of Chicken Muscle (1), Hamster Lung (2), Pig Heart(3), with GAPDH mouse mAb(2B8) diluted at 1:10,000.

Western Blot-GAPDH(REK0005)

Western blot analysis of Hela (1), Rat brain (2), Rabbit Muscle(3), Sheep Muscle(4), and Mouse brain (5) with GAPDH mouse mAb(2B8) diluted at 1:10,000.