Description |
β-actin Mouse Monoclonal Antibody(REK0001) |
Cat. No. |
REK0001 |
Immunogen |
synthetic peptide coupled to KLH. |
Concentration |
1ug/ul |
Specificity |
The β-actin mouse monoclonal antibody can detect endogenous β-actin proteins. |
MW |
N/A |
Substrate/Buffer |
Phosphate buffered saline (PBS) with containing 0.02% sodium azide. pH 7.2. |
Application |
WB 1:5000 (Optimal dilutions should be determined by the end user) |
Notes |
β-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Actin may not be stable in certain cells. For example, expression of β-Actin in adipose tissue is very low and therefore β-Actin should not be used as loading control for these tissues. |
Storage |
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
Purity |
Immunogen affinity purified |
Clonality |
Monoclonal |
Isotype |
IgG1 |
Alternative names |
actin antibody, b-actin antibody, beta-actin antibody |
Western Blot-β-actin(REK0001)-3Western blot analysis of 293T(1) , NIH3T3(2) with β-Actin mouse mAb(5B7) diluted at 1:10,000. |
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Western Blot-β-actin(REK0001)-2Western blot analysis of Hamster Kidney with β-Actin mouse mAb(5B7) diluted at 1:10,000. |
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IHC-β-actin(REK0001)IHC Staining of Human ovary tissue with β-Actin mouse mAb(5B7) diluted at 1:200 |
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Western Blot-β-actin(REK0001)Western blot analysis of Hela (1) Rat brain (2)Mouse brain (3) Chicken lung (4) and Rabbit testis (5)with β-Actin mouse mAb(5B7) diluted at 1:10,000. |