Flag-Tag Mouse Monoclonal Antibody(REK0047)
IF analysis of 293 cells transfected with a Flag-tag protein, using anti-FlagTag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-Flag)
Cat#： REK0047
Application： WB, IP, IF
Reactivlty： N/A
Conjugate： Unconjugated
Description： Flag-Tag Mouse Monoclonal Antibody
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IF analysis of 293 cells transfected with a Flag-tag protein, using anti-FlagTag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-Flag)
Cat#： REK0047
Application： WB, IP, IF
Reactivlty： N/A
Conjugate： Unconjugated
Description： Flag-Tag Mouse Monoclonal Antibody
Description：Flag-Tag Mouse Monoclonal Antibody(REK0047)
Cat. No.：REK0047
Immunogen：synthetic peptide coupled to KLH.
Concentration：1ug/ul
Specificity：The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag fusion proteins.
MW：N/A
Substrate/Buffer：Phosphate buffered saline (PBS) with containing 0.02% sodium azide. pH 7.2.
Application：WB 1:5000 (Optimal dilutions should be determined by the end user)
Notes：The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.
Storage：Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Purity：Immunogen affinity purified
Clonality：Monoclonal
Isotype：IgG1
Alternative names：DDDDK Tag antibody, DYKDDDDK Tag antibody
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