Description

β-actin Mouse Monoclonal Antibody(REK0004)

Cat. No.

REK0004

Immunogen

 synthetic peptide coupled to KLH.

Concentration

1ug/ul

Specificity

The β-actin mouse monoclonal antibody can detect endogenous β-actin proteins.

MW

N/A

Substrate/Buffer

Phosphate buffered saline (PBS) with containing 0.02% sodium azide. pH 7.2.

Application

WB 1:5000 

 (Optimal dilutions should be determined by the end user)

Notes

β-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Actin may not be stable in certain cells. For example, expression of β-Actin in adipose tissue is very low and therefore β-Actin should not be used as loading control for these tissues.  

Storage

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Purity

Immunogen affinity purified

Clonality

Monoclonal

Isotype

IgG1

Alternative names

actin antibody, b-actin antibody, beta-actin antibody