Description

Anti-Histone H3 (tri methyl K79) Mouse Monoclonal Antibody((REK1029)

Cat. No.

REK1029

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 50-100 of Human Histone H3, Tri methylated at K79.

Concentration

1ug/ul

Specificity

Histone H3 (Tri-Methyl K79) mAb detects endogenous levels of histone H3 only when tri-methylated at Lys79.

MW

15~ 17 kDa

Substrate/Buffer

Phosphate buffered saline (PBS) with containing 0.02% sodium azide. pH 7.2.

Application

WB 1:500-2,000 

IHC 1:200-500

(Optimal dilutions should be determined by the end user)

Notes

Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression.

Storage

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Purity

Immunogen affinity purified

Clonality

Monoclonal

Isotype

IgG

Alternative names

H3 histone antibody, HIST1H3A antibody, Histone cluster 1, H3a antibody