| Description | Anti-Histone H3 (mono-methyl K79) Mouse Monoclonal Antibody(REK1023) |
| Cat. No. | REK1023 |
| Immunogen | Synthetic peptide conjugated to KLH derived from within residues 1-100 of Human Histone H3, Mono-methylated at K79. |
| Concentration | 1ug/ul |
| Specificity | Histone H3 (mono-methyl K79) mAb detects endogenous levels of histone H3 only when mono-methylated at Lys79. |
| MW | 15~ 17 kDa |
| Substrate/Buffer | Phosphate buffered saline (PBS) with containing 0.02% sodium azide. pH 7.2. |
| Application | WB 1:500-2,000 IHC 1:200-500 (Optimal dilutions should be determined by the end user) |
| Notes | Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression. |
| Storage | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
| Purity | Immunogen affinity purified |
| Clonality | Monoclonal |
| Isotype | IgG |
| Alternative names | H3 histone antibody, HIST1H3A antibody, Histone cluster 1, H3a antibody |