Description

His -Tag Mouse mAb (REK0041)

Cat. No.

REK0041

Immunogen

 synthetic peptide coupled to KLH.

Concentration

1ug/ul

Specificity

The His tag antibody can recognize C-terminal, internal, and N-terminal His-tag fusion proteins.

MW

N/A

Substrate/Buffer

Phosphate buffered saline (PBS) with containing 0.02% sodium azide. pH 7.2.

Application

WB 1:5000 

 (Optimal dilutions should be determined by the end user)

Notes

polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells. 

Storage

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Purity

Immunogen affinity purified

Clonality

Monoclonal

Isotype

 IgG1

Alternative names

6 His tag antibody